Mining protein-protein interactions to study renal transporter regulation

Symposium — Sunday, April 23, 2024 — 10:30 AM - 12:30 PM — , Room W194B
Renal Section — Chair: Guangping Chen — Co-Chair: Dennis Brown

Transporter proteins become functionally active only after they move to the cell surface. During trafficking and recycling, the membrane proteins must interact with a host of other accessory proteins to perform their functions.  During the last decade, attention has been directed to important partner proteins and the protein-protein interaction network (the interactome). These networks play divergent roles in modulating transporter protein trafficking, phosphorylation, ubiquitylation, internalization, and degradation and their regulation is highly dynamic, selective, and complicated. Timely and selective interaction with specific binding proteins modulates signalling pathways and transporter protein function. Each accessory protein executes a particular role in assisting the membrane protein temporally and spatially, however, the mechanisms remain unclear. Mining protein-protein interaction is crucial for understanding transport proteins function and regulation. This symposium topic is new will attract a strong audience. Indeed, identifying key functional modulators and screening modulators/compounds to target specific binding proteins may have significant clinical impact.

Speakers

• Rab14 GTPase down-regulates UT-A1 urea transport activity through enhanced clathrin-dependent endocytosis
  Guangping Chen — Medicine, Emory University


• Regulation of ENaC trafficking by COMMD proteins
  Fiona McDonald — Physiology, University of Otago, School of Medical Sciences


• Mining the NKCC2 interactome to study Thick ascending limb function
  Paulo Caceres — Hypertension and Vascular Research Division, Henry Ford Hospital


• AQP2  a model protein for understanding the role of protein: protein interactions in protein trafficking
  Hanne Moeller — University of Aarhus, Department of Biomedicine